Atmospheric pressure plasma deposition (APPD) polymerization has more possible applications than low-pressure plasma polymerization due to its low cost and utilization versatility. One of the applications is protein immobilization, which can be used for biosensor fabrication. The conventional immobilization process of protein is complicated and regent consuming. On the contrary, the aerosol-assisted dielectric barrier discharge APPD (DBD-APPD) technique can deposit polyethylene (PE) film with OH functional groups and embed protein at the same time, making the immobilization process faster and avoids use of harmful reagents[1-3]. Hence, this work used DBD-APPD to immobilized protein onto substrate for biosensing application. As a result, we immobilized BSA with DBD-APPD, and the staining result shows that the biosening application is feasible.